Anatomical structure of vegetative and generative organs of Eremostachys laciniata (Lamiaceae

The anatomical structure of the shoots, roots, leaves and petals of the medicinal plant Eremostachys laciniata (L.) Bunge was studied to identify species-specific characteristics and diagnostic value.

It was revealed that: in basal and stem leaves, the cells of the upper epidermis are larger than in bracts. The number of vascular bundles in the petioles of the basal leaves of E. laciniata is three, with one large horseshoe-shaped bundle in the center and one small lateral rounded bundle on each side. The leaf blade of E. laciniata is amphistomatic. The stomatal apparatus is anomocytic, less often diacytic, stomatal cells are lenticular in shape. The stem is tetrahedral; the cut epidermis of the stem consists of straight-walled rectangular cells. Along the edge of the petal there are papillary projections and elongated two-cell simple trichomes with thick walls. The stem, petiole, leaves and petals have simple and glandular trichomes on the surface. The outside of the root is covered with a single-layer rhizoderm, the endoderm is not visible, and a plug is formed subendodermally. The primary xylem of the root is tetrarchic; the secondary xylem contains annual rings.

The research results can be used to develop regulatory documentation and introduce medicinal plant raw materials and preparations from E. laciniata into medical and pharmaceutical practice.

1. Anisimova A. G., Demyanova E. I. 2007. Morphological and anatomical features of sexual forms of Origanum vulgare (Lamiaceae). Plant resources 43(1): 36–45. (In Russ.).

2. Barykina R. P. 2004. Spravochnik po botanicheskoj mikrotekhnike. Osnovy i metody. [Handbook of Botanical Microtechnics. Fundamentals and methods.] Mосква: 312 p. (In Russ.).

3. Chebotareva A. A., Dikun N. A. 2018. Morphological and anatomical features of Thymus marschal lianus herb. Aktual'nye problemy ekologii proirodopol'zovaniya v sovremennyh usloviyah: Materialy Mezhdunarodnoj nauchno-prakticheskoj konferencii [Actual problems of environ mental management in modern conditions: Materials of the International Scientific and Practical Conference.]. Kirov: 210–212. (In Russ.).

4. Delazar A., Habibi A. H., Mohammadi O., Afshar F.H., Nahar L., Modarresi M., Nazemiyeh H. and Sarker S. 2009. Evaluation of analgesic activity of Eremostachys laciniata in mice. Journal of Natural Remedies 9:1–7.

5. Delazar A., Sarker S. D., Nahar L., Barzegar Jalali S., Modaresi M., Hamedeyazdan S., Babaei H., Javadzadeh Y., Asnaashari S., and Bamdad Moghadam S. 2013. Rhizomes of Eremostachys laciniata: isolation and structure elucidation of chemical constituents and a clinical trial on inflammatory diseases. Advanced Pharmaceutical Bulletin 3: 385–393.

6. Delova G. V., Guskova I. N. 1974. Antibacterial and antifungal properties of essential oils of some species of Lamiaceae. Comprehensive study of useful plants of Siberia. Novosibirsk: 131–146. (In Russ.).

7. Erdemoglu N., Turan N. N., Cakoco I., Sener B., Aydon A. 2006. Antioxidant activities of some Lamiaceae plant extracts. Phytotherapy Research 20: 9–13.

8. Flora SSSR. T. 21 [Flora of the USSR. Vol. 21]. 1945. Мoscow, Leningrad: 704 p. (In Russ.).

9. Grossgeim А. А. 1940. Flora Kavkaza. T. 7 [Flora of the Caucasus. Vol. 7]. Leningrad: 284 p. (In Russ.).

10. Khan S., Nisar M., Rehman W. and Nasir F. 2010. Anti-inflammatory study on crude methanol extract and different fractions of Eremostachys laciniata. Pharmaceutical Biology 48: 1115– 1118.

11. Konyaeva E. A., Alentyeva O. G. 2019. Morphological and anatomical study of the Dracocephalum moldavica L. introduced into the VILAR. Issues of quality assurance of medicines 26(4): 4–(In Russ.).

12. Konyaeva E. A., Alentyeva O. G., Saibel O. L., Dargaeva T. D., Potanina O. G., Mizina P. G., Sidelnikov N. I. 2020. Morfologo-anatomicheskie priznaki nekotoryh novyh vidov lekarstvennogo rastitel'nogo syr'ya [Morphological and anatomical features of some new types of medicinal plant raw materials.]. Moscow: 211 p. (In Russ.).

13. Modaressi M., Delazar A., Nazemiyeh H., Fathi-Azad F., Smith E., Rahman M. M., Gibson S., Na har R., Sarkaer S. 2009. Antibacterial iridoid glucosides from Eremostachys laciniata. Phytotherapy Research 23: 99–103.

14. Murtazaliev R. A. 2009. Fam. Lamiaceae. Konspekt flory Dagestana. T. 3 [Conspectus of the flora of Dagestan. Vol. 3]. Makhachkala: 304. (In Russ.).

15. Nikitina A. S., Shevchuk O. M., Feskov S. A., Nikitina N. V., Dubovaya M. N. 2018. Morphologi cal-anatomical and morphometric study of the herb Monarda didyma L. from the collection of the Nikitsky Botanical Garden. Perspektivy lekarstvennogo rastenievedeniya: Materialy Mezhdunardnoj nauchnoj konferencii, posvyashchennoj 100-letiyu professora. A.I. SHretera [Prospects of medicinal plant science: Materials of the International Scientific Conference, dedicated to the 100th anniversary of Prof. A.I. Schroeter]. Moscow: 490–496. (In Russ.).

16. Nisar M., Khan S., Dar A., Rehman W., Khan R., Jan I. 2010. Antidepressant screening and flavonoids isolation from Eremostachys laciniata (L) Bunge. African Journal of Biotechnology 10: 1696–1699.

17. Said O., Khalil K., Fulder S., Azaizeh H. 2002. Ethnopharmacological survey of medicinal herbs in Israel, the Golan heights and the west bank region. Journal of Ethnopharmacology 83: 251– 265.

18. Samylina I. A., Anosova O. G. 2007. Farmakognoziya: Atlas: uchebnoe posobie T. 1. [Pharmacog nosy: Atlas: textbook. Vol. 1.]. Moscow: 192 p. (In Russ.).

19. Serebryanaya F. K. 2014. Morphological and anatomical study of Pushkin's Ziziphora (Ziziphora puschkinii Adams) of the Lamiaceae family Lindl. Pharmacy and Pharmacology 4(3): 44–50. (In Russ.)

20. Tananykina E. K., Polovetskaya O. S. 2020. Comparative morphological and anatomical analysis of Mentha L. raw materials of various varieties. Modern Science 6(2): 20–25. (In Russ.)

21. The Plant List. Version 1.1. 2013. http://www.theplantlist.org/

22. Uritu C. M., Mihai C. T., Stanciu G.-D., Dodi G., Alexa-Stratulat T., Luca A., Leon-Constantin M. M., Stefanescu R., Bild V., Melnic S. et al. 2018. Medicinal Plants of the Family Lamiaceae in Pain Therapy: A Review. Pain Research and Management P. 1–44. https://doi.org/10.1155/2018/7801543

23. Zaitsev G. M. 1984. Matematicheskaya statistika v eksperimental'noy botanike [Mathematical statistics in experimental botany.]. Moscow: 424 p. (In Russian)

24. Zvezdina E. V., Dayronas Zh. V., Bochkareva I. I., Zilfikarov I. N., Babaeva E. Yu., Ferubko E. V., Guseynova Z. A., Serebryanaya F. K., Kaibova S. R., Ibragimov T. A. 2020. Representatives of the Fam. Lamiaceae Lindl as sources of medicinal plant raw materials for the production of neurotropic drugs (review). Pharmacy and pharmacology 8(1): 4–28. https://doi.org/10.19163/2307-9266-2020-8-1-4-28