Phylogenetic relationships of Rosa oxyodon Boiss.
https://doi.org/10.33580/24092444_2022_1_19
Abstract
The main purpose of this work was to identify a possible way of speciation of the Caucasian endemic R. oxyodon Boiss. In this regard, an attempt was made to study the genetic relationships of R. oxyodon with related species R. pendulina, R. majalis, and R. donetzica by sequencing the sequence of the trnL-trnF chloroplast spacer. The sample size for chloroplast DNA analysis was 48 samples. DNA isolation was carried out by the STAB method from dried leaves. In all studied samples, the sequence of the chloroplast intergenic spacer trnL-trnF was sequenced. The sequenced sequences of the trnL-trnF chloroplast spacer were manually aligned using the BioEdit v7.0.5 program. Data analysis was carried out using the statistical parsimony method implemented in the TCS v. 1.21. The analysis included 83 sequences. The alignment length was 293 positions. As a result of the work done, 18 haplotypes were identified, 7 of which were absent among the samples and were hypothetical extinct. The remaining haplotypes were distributed as follows: 1) R. pendulina: A, B, C, D, E; 2) R. majalis: F, L, M; 3) R. oxyodon: A, F, G, H, I; 4) R. donetzica: E, F.
R. oxyodon is characterized by haplotypes A and F, genealogically distant from each other and occurring in R. pendulina, R. majalis, and R. donetzica, respectively. Such a distribution of haplotypes in the three species under consideration, including disjunctions in the distribution of haplotype A, suggests the hybrid origin of R.oxyodon as a result of multiple reciprocal (at least two) hybridizations between R. pendulina and R. majalis.
About the Author
B. A. Ramazanova
Mountain Botanical Garden of Dagestan Federal Research Centre of Russian Academy of Sciences
Russian Federation
Russian Federation
Ramazanova Bayzanat Abakarovna, Candidate of Biology, Junior researcher of the Laboratory of introduction and genetic resources of woody plants,
45, M. Gadzhieva St., Makhachkala 367000.
References
1. Avise J. C., Arnold J., Ball R. et al. 1987. Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Annu. Rev. Ecol. Syst. 18: 489–522
2. Fer Т., Vasak P:, Vojta J., Marhold K. 2007. Out of the Alps or Carpathians? Origin of Central European populations of Rosa pendulina. Preslia 79: 367–376
3. Shancer I. A. 2011. Gibridizaciya, polimorfizm i filogeneticheskie otnosheniya vidov roda Rosa L. Avtoref. Dokt. Diss. [Hybridization, polymorphism and phylogenetic relationships of species of the genus Rosa L. Abstr. Doct Diss.]. Moscow: 41 p (In Russ.).
4. Galushko A. I. 1980. Flora Severnogo Kavkaza [Flora of the North Caucasus. Vol. 2]. Rostov: 351 p. (In Rus.).
5. Buzunova I. O., Kamelin R. V. 2004. Species of the genus Rosa L. (Rosaceae) of the section Cinnamomeae DC. in the flora of the Caucasus. News system. nizsh. rast. 36: 112–122 (In Rus.).
6. Doyle J. J., Doyle J. L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. 1987. Phytochemical Bulletin. 19: 11–15
7. Hall A. T. 1999. BioEDit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium 41: 95–98
8. Templenton A. R., Crandall K. A., Sing C. F. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data III. Cladogram estimation. 1992. Genetics 132: 619–633
9. Clement M., Posada D., Crandall K. A. TCS: a computer program to estimate gene genealogies. 2000. Molecular Ecology 9: 1657–1659
10. Lepehina A. A. 1971. Opredelitel' derev'ev i kustarnikov Dagestana. [Determinant of trees and shrubs of Dagestan]. Mahachkala: 243 p. (In Russ.).
11. Galushko A. I. 1959. Shipovniki srednej chasti severnogo sklona Bol'shogo Kavkaza i ih hozyajstvennaya cennost'. Avtoref. Cand. Diss. [Rosehip and the middle part of the northern slope of the Greater Caucasus and their economic value. Abstr. Cand. Diss.]. Moscow: 26 p. (In Russ.).
12. Kamelin R. W. 1996. Asian mountain elements in the flora of the Caucasus. Flora i rastitel'nost' Altaya: tr. YUzhno-Sibirskogo bot. sada: 5–22. (In Rus.).
13. Shkhagapsoev S. H., Starikiva N. V. 2002. Analiz estestvennoj dendroflory Kabardino-Balkarii. [Analysis of the natural dendroflora of Kabardino-Balkaria]. Nalchik: 113 p. (In Rus.).
14. Shancer I. A., Abakarova B. A. 2012 Proiskhozhdenie Rosa oxyodon i plejstocenovye kontakty mezhdu Karpatami i Kavkazom. Biogeografiya: metodologiya, regional'nyj i metodologicheskij aspekty [The origin of Rosa oxyodon and Pleistocene contacts between the Carpathians and the Caucasus. Biogeography: methodology, regional and methodological aspects]. Moscow: 248–252. (In Rus).
15. Hrzhanovsky V. G. 1958. Rosi (Roses). Moscow. 496 p. (In Rus.).
Review
For citations:
Ramazanova B.A. Phylogenetic relationships of Rosa oxyodon Boiss. Botanical Journal of the North Caucasus. 2022;(1):19-26. (In Russ.) https://doi.org/10.33580/24092444_2022_1_19
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