Genetic variability of wild rose (Rosa oxyodon) by ISSR-markers
https://doi.org/10.33580/24092444_2024_2_46
Abstract
In practice, it is difficult to distinguish closely related species from each other, which is usually associated with high intraspecific polymorphism. The aim of this work is to assess the variability of the species R. oxyodon, R. prokhanovii and R. sosnovskyana. At the first stage, 42 morphological characters were analyzed. The same samples were studied in relation to the polymorphism of ISSR markers and the variability of the chloroplast spacer trnL-trnF. Analysis of the morphological characters of R. oxyodon, R. prokhanovii and R. sosnovskyana by the principal coordinates method showed the impossibility of their strict differentiation as independent and equivalent species. The same result was obtained when analyzing the polymorphism of 115 ISSR markers. Data on the variability of the chloroplast spacer trnL-trnF of the above-mentioned species also showed that the existing polymorphism is not associated with division into three species.
About the Author
B. A. RamazanovaRussian Federation
Ramazanova Bayzanat Abakarovna, Candidate of Biology, Junior researcher of the Laboratory of introduction and genetic resources of woody plants
367000, Makhachkala, M. Gadzhieva St., 45
References
1. Anderson E. C., Thompson E. A. 2002. A model-based method for identifying species hybrids using multilocas genetic data. Genetics 160: 1217–1229.
2. Buntjer J. B. 2000. Cross Checker: computer assisted scoring of genetic AFLP data. Plant & Animal Genome VIII Conference. San Diego: 945–959.
3. Buzunova I. O., Kamelin R. V. 2004. Species of the genus Rosa L. (Rosaceae) section Cinnamomeae DC. in the flora of the Caucasus. Novosti Sistematiki Vysshikh Rastenii 36: 112–122. (In Russ.).
4. Clement M., Posada D., Crandall K. A. 2000. TCS: a computer program to estimate gene genealogies. Molecular Ecology 9: 1657–1659.
5. Doyle J. J., Doyle J. L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: P. 11–15.
6. Falush D., Stephens M., Pritchard J. 2007. Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molec. Ecol. Notes 7: 574–578.
7. Fedorova A. V. 2014. Sistematika i geografiya vidov roda Rosa L. sekc. Gallicanae DC [Taxonomy and geography of species of the genus Rosa L. section Gallicanae DC. Abstr. Cand. Diss.]. Moscow: 21 p. (In Russ.).
8. Gadzhieva S. V. 2020. Diversity of Wild Pomegranate (Punica granatum L.) Genotypes in Azerbaijan Using ISSR Markers. Vestnik KrasSAU 3: 20–28.
9. Grant V. 1984. Vidoobrazovanie u rastenij. [Speciation in plants]. Moscow: 528 p. (In Russ.).
10. Grusheckaya Z. E., Nikitinskaya T. V., Kubrak S. V., Dzyuban O. V., Kukhareva L. V., Poliksenova V. D., Titok V. V., Lemesh V. A., Parfenov V. I., Khotyleva L. V. 20012. Using ISSR analysis to study intra- and interspecific genetic polymorphism of different taxa of higher plants. Vestnik BGU 3: 50–56. (In Russ.).
11. Hall A. T. 1999. BioEDit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Oxford Unifersity Press. Nucleic Acids Symposium Series 41: 95–98.
12. Hammer O., Harper D.A.T., Ryan P. D. 2001. PAST: Paleontological statistics software package for education and data analysis. Paleontologia Electronica 1: 49.
13. Kalendar R. N., Glazko V. I. 2002. Types of molecular genetic markers and their application. Fiziologiya i biohimiya kul'turnyh rastenij 4: 279–293. (In Russ.).
14. Kashin A. S., Kritskaya T. A., Shantzer I. A. 2016. Genetic polymorphism of Tulipa gesneriana L. according to ISSR labeling data. Genetika 10: 1134–1145. (In Russ.).
15. Kolobov E.S. Rose hips of Dagestan. Avtoref. Cand. Diss. [Shipovniki Dagestana. Abstr. Cand. Diss.] 1985. Moscow: 11 p. (In Russ.).
16. Kumar M., Mishra G. P., Singh R., Kumar J., Naik P. K., Singh S. B. 2009. Correspondence of ISSR and RAPD markers for comparative analysis of genetic diversity among different apricot genotypes from cold arid deserts of trans-Himalayas. Physiology and Molecular Biology of Plants 3: 225–236.
17. Kutsev G. M. 2009. Fragmentarnyj analiz DNK rastenij: RAPD, DAF, ISSR. [Fragmentary analysis of plant DNA: RAPD, DAF, ISSR]. Barnaul: 164 p. (In Russ.).
18. Neve G., Meglecz E. 2000. Microsatellite frequencies in different taxa. Trends in Ecology & Evolution 15: 3321–3323.
19. Ramazanova B. A. 2012. Struktura, resursnyj potencial dagestanskih populyacij i filogeneticheskie svyazi shipovnika ostrozubogo (Rosa oxyodon Boiss.). [Structure, resource potential of Dagestan populations and phylogenetic relationships of Rosa oxyodon Boiss. Abstr. Cand. Diss.]. Stavropol: 20 p. (In Russ.).
20. Saki S., Bagheri H., Deljou A., Zeinalabedini M. 2016. Evaluation of genetic diversity amongst Descurainia sophia L. genotypes by inter-simple sequence repeat (ISSR) marker. Physiology and Molecular Biology of Plants 22: 97–105.
21. Shahi-Gharahlar A., Zamani Z., Fatahi R., Bouzari N. 2011. Estimation of genetic diversity in some Iranian wild Prunus subgenus Cerasus accessions using inter-simple sequence repeat (ISSR) markers. Biochem Syst Ecol 4–6: 826–833.
22. Shancer I. A., Voilokova V. N. 2008. How many species related to Rosa majalis grow in the European part of Russia? Botanicheskii zhurnal 93: 1690–1704. (In Russ.).
23. Taberlet P, Gielly L, Pautou G, Bouvet J. 1991. Universal primers for amplification of three noncoding regions of chloroplast DNA. Plant Molecular Biology 17: 1105–1109.
24. Tahtadzhyan A. L. Zhizn' rastenij. [Life of Plants]. Vol. 1. 1974. Moscow: 49–57. (In Russ.).
25. Templenton A. R., Crandall K. A., Sing C. F. 1992. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data III. Cladogram estimation. Genetics 132: 619–633.
26. Tharwat R., Mazen N., Hafez M. 2018.Genetic Diversity of Rosa Damascena Mill. in Latakia Province as Reveled by ISSR Analysis. SSRG International Journal of Agriculture&Environmental Science 6: 18–22.
27. Zahid N. S., Vikas S., Rafiq A. S., Neha S., Baby S., Fahad A., Hamed A., Javid I. M. 2021. Genetic diversity analysis and population structure in apricot (Prunus armeniaca L.) grown under north-western Himalayas using ISSR markers. Saudi Journal of Biological Sciences 10: 5986–5992.
28. Zavadskiy K. M. 1968. Vid i vidoobrazovanie. [Species and Speciation]. L: 404 p. (In Russ.).
Review
For citations:
Ramazanova B.A. Genetic variability of wild rose (Rosa oxyodon) by ISSR-markers. Botanical Journal of the North Caucasus. 2024;(2):46–56. (In Russ.) https://doi.org/10.33580/24092444_2024_2_46